Guidance on Analytical Procedures and Validation for Deuterated Molecules

Dear Experts,

Greetings!

I am seeking guidance on analytical methodologies and method validation strategies for determining isotopic purity, isotopic enrichment, and deuterium content (%D) in deuterated compounds.

If available, could you please share any relevant:

  • USP/Ph. Eur./JP or other pharmacopeial guidance

  • Regulatory guidance documents

  • Scientific publications or literature references

  • Analytical protocols or validation approaches

  • Practical experiences and best practices

Your valuable suggestions and insights would be highly appreciated.

Thank you in advance for your support.

Best regards,

Gautam G. Halder